Semen Leukocyte Staining Solution (Peroxidase Assay)
Detecting Leukocytospermia: The Clinical Role of the Peroxidase Assay in Male Tract Infection
What it is
The Semen Leukocyte Staining Solution uses a cytochemical Peroxidase Assay to identify polymorphonuclear leukocytes (white blood cells) in semen. Cells containing peroxidase react with the solution to turn a distinct brown color, while immature germ cells (round cells) remain unstained.
Why it is Essential
Under a standard microscope, Round Cells (immature sperm) look identical to Leukocytes. Diagnosing Infection: High leukocyte counts (>1 million/mL) define Leukocytospermia, a sign of silent infections (e.g., Prostatitis). Reducing ROS Damage: Leukocytes are a major source of Reactive Oxygen Species (ROS), which cause lipid peroxidation of the sperm membrane and lead to DNA damage. Quantify oxidative stress with the NBT Assay; assess DNA integrity with the SCD Assay.
How it Aligns with Standards
The WHO 6th Edition recognizes the Peroxidase Assay as the only manual method for the definitive diagnosis of leukocytospermia. Our kit ensures high sensitivity, allowing labs to differentiate between infection-based infertility and maturation-based "round cell" issues.
References
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