Sperm Vitality Staining Solution (Eosin-Nigrosin Staining)
Vitality vs. Motility: Differentiating Live and Dead Sperm with Eosin-Nigrosin Staining
What it is
The Sperm Vitality Staining Solution uses the Eosin-Nigrosin method to assess the membrane integrity of spermatozoa. Eosin is a "cell-excluded" dye; it only enters sperm with a damaged cell membrane (dead sperm), staining them pink. Nigrosin acts as a dark background stain to provide sharp contrast for the white (alive) sperm.
Why it is Essential
Vitality testing is clinically mandatory when total motility is below 40%. Differentiating Necrozoospermia: It helps determine if immobile sperm are actually dead (Necrozoospermia) or alive but structurally defective (Asthenozoospermia). ICSI Support: For patients with zero motility, vitality staining is the only way to ensure that "live" sperm are selected for injection.
How it Aligns with Standards
The WHO recommends Eosin-Nigrosin as the Gold Standard because it is non-toxic to the technician and provides a permanent slide. Our solution is calibrated for neutral pH and correct osmolarity to prevent "artifactual" cell death during the staining process.
References
Related Product: Sperm Vitality Staining Solution (Eosin-Nigrosin Staining) → View Product