When clinical scenarios require the highest resolution of DNA damage assessment, the Comet Assay is the definitive choice. Unlike the SCD Assay and other standard screening tests, the Comet Assay utilizes single-cell electrophoresis to migrate fragmented DNA out of the sperm nucleus, creating a "comet-like" tail that can be quantified with high precision.
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Sperm DNA Fragmentation Test Kit (Comet Assay)
The Gold Standard in DNA Damage Sensitivity: Single-Cell Gel Electrophoresis (Comet Assay)
The Ultimate Sensitivity:
Why Comet Assay?
Comprehensive Detection: Recognized by the WHO 6th Edition as a specialized test, it is the only assay capable of differentiating between single-strand and double-strand DNA breaks.
Quantitative Precision: Can be analyzed qualitatively via fluorescence microscopy or quantitatively using digital analysis software for an objective Tail Moment calculation.
ICSI & IVF Selection: Critical for evaluating sperm DNA fragmentation in severe male infertility and high-stakes ART cycles.
References
- WHO (2021). WHO Laboratory Manual for the Examination and Processing of Human Semen, 6th Edition.
- Simon L, et al. (2011). Sperm DNA damage measured by the alkaline Comet assay as an independent predictor of male infertility and IVF success. Fertility and Sterility.
- Ribas-Maynou J, et al. (2013). Comprehensive analysis of sperm DNA fragmentation by five different assays. Andrology.